Chromomycin A3 as a fluorescent probe for flow cytometry of human gynecologic samples.
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چکیده
منابع مشابه
Evaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
متن کاملP-11: DHR123: A Suitable Fluorescent Mitochondrial Probe for Assessment of H2O2 in Human Spermatozoa
Background: The objective of present study was to assess potential of DHR123 (dihydrorhodamine 123) fluorescent probe for measurement of H2O2 produced by human spermatozoa and comparing the results with DCFH-DA (2', 7'-dichlorodihydrofluorescein diacetate). Materials and Methods: Fluorescent intensity and percentage R123 and DCF positive sperm measured by flow cytometry. The suitable ...
متن کاملevaluation of protamine level in human sperm samples using chromomycin a3 and aniline blue staining
background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...
متن کاملFluorescent erythrocyte ghosts as standards for quantitative flow cytometry.
We report here a quick and inexpensive method for preparing standards of known fluorochrome content for calibration and quantitation of flow cytometry fluorescence signals. Erythrocyte ghosts prepared by hypotonic lysis are filled with solutions containing fluorescently labeled dextran. Standards prepared by this technique have a narrow range of fluorescence and a linear response of fluorescenc...
متن کاملFlow cytometry of fluorescent proteins.
Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1977
ISSN: 0022-1554,1551-5044
DOI: 10.1177/25.7.70448